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Nditioned medium, the nonadherent cells were washed off again, resulting in a population of cells with predominantly macrophage (>99 ) morphology as determined with the modified Wright's stain. Adherent AM monolayers were infected with J2 virus supernatants mixed with DMEM conditioned media and polybrene. After 24 h, excess virus was removed and cells were exposed to a second round of J2 infectio
Rther decreased the yolk and are recommended for 2D gel electrophoresis. The samples were frozen in liquid nitrogen or processed directly for Western blotting or 2D gel electrophoresis.Due to the lack of calcium the cells dissociate during the deyolking. Addition of 2.7 mM CaCl2 to the deyolking buffer preserves bigger cell clusters or cell sheets. Deyolking was only slightly less efficient in th
Rther decreased the yolk and are recommended for 2D gel electrophoresis. The samples were frozen in liquid nitrogen or processed directly for Western blotting or 2D gel electrophoresis.Due to the lack of calcium the cells dissociate during the deyolking. Addition of 2.7 mM CaCl2 to the deyolking buffer preserves bigger cell clusters or cell sheets. Deyolking was only slightly less efficient in th
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He cerebellum and brain stem in Down's syndrome and Alzheimer's disease: a light microscopical analysis. Acta Neuropathol 1993, 85(5):542-552. 83. Tong M, de la Monte SM: Mechanisms of ceramide-mediated neurodegeneration. J Alzheimers Dis 2009, 16(4):705-714. 84. Hietanen E, Bartsch H, Ahotupa M, Bereziat JC, Bussacchini-Griot V, Cabral JR, Camus AM, Laitinen M, Wild H: Mechanisms of fat-related
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Hobbies Are What Make Individuals who They are



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Hrough autophagy. Immunity. 2007;27(1):11?1. 93. Pua HH, Dzhagalov I, Chuck M, Mizushima N, He YW. A critical role for the autophagy gene Atg5 in T cell survival and proliferation. J Exp Med. 2007;204(1):25?1.94. Hubbard VM, Valdor R, Patel B, Singh R, Cuervo AM, Macian F. Macroautophagy regulates energy metabolism during effector T cell activation. J Immunol. 2010;185(12):7349?7. 95. Jia W, He Y
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